In this technique overies are stimulated by injecting exogenous hormones for faster growth of more number of follicles and ovulation of more ova per estrous cycle. After insemination of donar with superior bull semen more embryos of elite parents can be produced per estrous cycle. These embryos are flushed, graded and than transferred in foster mothers of low genetic worth for completing gestation. Thus superior animals can be multiplied more quickly.Buffalo is a comparatively poor responder to superovulatory treatment. While zebu cattle and their crosses give about 4.5 embryos per flush, the best results in buffaloes indicate only 1.45- 2.0 embryos per flush. The poor responses are attributed to inherent endocrine diversity, the characteristics of follicular population and ovrian folliculogenesis, hormonal profile during the superovulatory cycle, and failure to recover embryos due to different embryos transport speeds or due to asynchrony wih uterine or ovarian events.

The superovulatory response in buffaloes is influenced by level of nutrition, season, parity, individual response, hormonal preparation and dose, day of treatment, day of collection of embryos etc. the effect of season on embryo recovery indicated that embryos recovered in summer are less in number and are of poor quality but in winter embryos recovered are more in number as well as of good quality. Animals in first and second parity yield more number of transferable embryos than multiparous buffaloes. In spite of poor superovulatory response in buffaloes, this technique can be effectively used for production of superior bulls for artificial insemination program.